Hold the pipette so the "grippy finger rest" rests on your index finger. Set the volume within the range specified for the pipette. General guidelines and Pipetting techniques General guidelinesĬheck your pipette at the beginning of your working day for dust and dirt onthe outside. The boiling point of some liquids can also change to quite close to room temperature, increasing the evaporation loss dramatically. The air pressuredecreases at higher altitudes and the conversion factor Z decreases as well. The geographic altitude affects the accuracy through the air pressure. Typically, the density of water is 0.996 kg/dm3, for ethanol 0.79 kg/dm3 and for sulfuric acid (H2SO4) 1.85 kg/dm3. The density varies according to thetemperature and air pressure. If the temperature of the liquid, pipette and air is the same, the accuracy is not significantly affected.ĭensity is the mass/volume ratio of the liquid. The figure below shows the change in volume when the liquid has a different temperature than the pipette and air. The most important factor in pipetting accuracy is the liquid temperature. To empty the tip completely the operating button is pressed to the second stop (blow out).įactors affecting the accuracy of Air Displacement Pipettes:
When the operating button is pressed to the first stop again, the air dispensesthe liquid.After immersing the tip into the liquid, the operating button is released.This creates a partial vacuum and the specified volume of liquid is aspiratedinto the tip.When the operating button is pressed to the first stop, the piston expels thesame volume of air as indicated on the volume setting.
The piston moves to the appropriate position when the volume is set.How does an Air Displacement Pipette work? However, conditions such as temperature, atmospheric pressure as well as the specific gravity and viscosity of the solution may have an effect on the performance of air displacement pipettes. Please note our licence (freeware) for this product before you use the software.Air Displacement Pipette, used for standard pipetting applications, is highly accurate. Pay as much as you want! This software can be downloaded for free and you can use it without any restrictions for non-commercial purposes. Windows, Linux and macOS Linux, Windows and macOS macOS, Windows and Linux More languages.ġ.75 MB to 3.64 MB - depending on the Program Version English, Dansk (Danish), Deutsch (German), Español (Spanish), Français (French), Italiano (Italian), Nederlands (Dutch), Polski (Polish), Português do Brasil (Brazilian Portuguese), Русский (Russian), العربية (Arabic), 日本語 (Japanese) and 简体中文 (Simplified Chinese).
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